Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
120 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
UNSPSC |
41115709 |
Application |
Polyaromatic Hydrocarbon |
Brand |
Waters |
Product Type |
Columns |
Units per Package |
1 pk |
Waters C18 Column, 120Å, 5 µm, 4.6 mm X 250 mm, 1/pk
As the most frequently monitored environmental contaminants, Polynuclear Aromatic Hydrocarbons (PAHs) are usually recommended for use with methods of analysis found in compendia for air, drinking water, wastewater, solid waste, and food analysis. Most of these methods specify HPLC with UV and fluorescence detection as the recommended analytical procedure. The Waters C18 Column for PAH has been optimized for HPLC analysis of PAHs.
The United States Environmental Protection Agency lists 16 PAH compounds as target pollutants. The Waters C18 Column for PAH achieves baseline resolution and excellent peak symmetry across all 16 of these target analytes in under 25 minutes, while employing simple water; acetonitrile binary gradient. The resolving power in these PAH Columns allows for superior peak identification and quantitation for PAHs. These columns can also resolve the 2 additional compounds included in Florida Administrative Code 17.700.
Columns are shipped individually and feature an inner diameter of 4.6 mm with a length of 250 mm, as well as a particle size of 5 µm. Operating limits include a pH range of 2 pH to 8 pH, a maximum pressure of 6000 psi (415 bar), and a recommended temperature range of 20 to 50 degrees Celsius.
Waters PAH columns and similar lab equipment are manufactured and packed under highly controlled conditions, and each column passes stringent tests before being accepted for shipment. Each column includes a final Certificate of Analysis based on these tests. To perform further testing, you can use the Waters Reversed-Phase QC Reference Material. These columns are backed by a world-class ISO 9002 registered documentation trail in accordance with Waters’ commitment to reproducibility between columns and batches.
How Should I Store Waters PAH Columns?
When storing for periods longer than 4 days, store Waters PAH columns in 100% acetonitrile. Columns should not be stored in buffered, acidic, or basic eluents. If the mobile phase contained a buffer salt, the column should be flushed using 10 column volumes of HPLC grade water, then replaced with 100% acetonitrile. Be sure to completely seal columns to avoid evaporation and drying out of the bed. Changes to retention time, resolution, or backpressure may indicate column contamination and potential improper storage protocol.