Simultaneous Quantification of Saxagliptin, 5-Hydroxy Saxagliptin, and Dapagliflozin in Human Plasma Using SPE and UPLC-MS/MS Analysis

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PMN Rajesh, Banda Jagadeesh, Mary Lame, and Dr. Padmakar Wagh
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This application details the simultaneous extraction and quantification of Saxagliptin, 5-Hydroxy Saxagliptin, and Dapagliflozin from plasma using a simple, mixed-mode SPE sample preparation in the μElution format, and subsequent UPLC-MS/MS analysis.

The analytical sensitivity and excellent performance of this method can be attributed to use of a highly specific extraction using mixed-mode SPE, high resolution chromatographic separation with an ACQUITY HSS C18 Column on an ACQUITY UPLC I-Class System, and high MS sensitivity of the Xevo TQ-S micro Mass Spectrometer. With its simplicity and excellent performance, this fit-for-purpose method shows promise to support drug research and development as well as clinical research.

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