Quantitative Determination of Plant Growth Regulators (PGRs) in Grape Matrix Using the Xevo TQD

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Bhaskar K, Rajesh PMN and Dr. Padmakar Wagh
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Application Notes
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Technical Notes
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In this technology brief, eight different classes of synthetic plant growth regulators were investigated using Waters tandem quadrupole Xevo TQD Technology. Using a simple acetonitrile dilution followed by liquid-liquid extraction, all pesticides were successfully extracted from the grape matrix and analyzed in an 8.0 minute run. A pentflurophenyl bonded phase column was selected for HILIC separation with an MS friendly mobile phase consisting of 0.1% aqueous formic acid with ammonium acetate and an acetonitrile gradient with electrospray positive ionization mode. The Xevo TQD coupled with the ACQUITY UPLC I-Class System achieves mg/kg detection limits for thiocyclam, abamectin, azadirachtin, cartap hydrochloride, chlormequat chloride, 6 benzaldehyde, mepiquat, and nereistoxin.

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