Exploring Selectivity Benefits of Fluorinated-Phenyl Stationary Phases

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Mia Summers and Kenneth J. Fountain
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Application Notes
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Application Notes
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In this application note, we compare the use of fluorinated-phenyl phases, including the ACQUITY UPLC HSS PFP and ACQUITY UPLC CSH Fluoro-Phenyl Columns, with C18 and Phenyl stationary phases. For method development, column screening is facilitated with automation, using the ACQUITY UPLC H-Class System with a four-position Column Manager. Screening columns with wide selectivity ranges, understanding interactions between the analytes and stationary phases, and early identification of the best column for a desired separation can save a tremendous amount of time and effort when developing a new method.

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