Advanced Topics In Peptide Mapping: Developing Reproducible High Resolution Separations

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P. Jayaraman, S. Koza, C. Reed, H. Yang, E. Chambers
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The peptide mapping of proteins is a fundamental tool used that
has been applied to both the proteomics-based discovery of new
biotherapeutic proteins and for monitoring the modification and
degradation of those proteins as they are developed and
commercialized1. When developing a reproducible and informative
peptide map the enzymatic digestion protocol and the separation
of the resultant peptides need to be optimized. We will focus on
column selection considerations for reversed-phase separation
based peptide mapping, however, many of these column selection
considerations can also be directly applied to the selection of
columns for LC-MS based proteomics studies and synthetic
peptide purity analyses.


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