We describe a sensitive LC-MS/MS quantification method for the robust analysis of highly polar pesticides in food commodities without the need of derivatization.
Using the ACQUITY UPLC System coupled to the Xevo TQ-XS Triple Quadrupole Mass Spectrometer, we achieved sub-parts per billion detection (ppb) limits for glyphosate, glufosinate, and aminomethylphosphonic acid (AMPA).
Traditionally, derivatization has been used to allow for chromatographic retention on reversed-phase columns. While good sensitivity and repeatability have been reported with this technique, there is an interest in direct analysis to avoid sample preparation and derivatization.
The sensitivity of the Xevo TQ-XS provides excellent limits of detection, suitable for monitoring maximum residue limit (MRL) compliance for these challenging analytes and good quantitative performance, in terms of precision and calibration characteristics in compliance with SANTE 11954/2015 guidelines.