UPLC-FLR Method Development of 2-AB Labeled Glycan Separation in Hydrophilic Interaction Chromatography (HILIC)

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Joomi Ahn, Ying Qing Yu, and Martin Gilar
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Application Notes
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Application Notes
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Monoclonal antibody (mAB) drug development is an active area in biotherapeutic research. Hydrophilic interaction chromatography (HILIC) with fluorescence detection is one approach to profile glycosylation patterns in recombinant mAbs. As shown in this application note, the Waters ACQUITY UPLC/FLR System used with Glycan Separation Technology Columns achieves highly-resolved glycan separations. This provides significant benefits to high-throughput glycosylation monitoring and profiling.

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