ACQUITY UPLC®系統結合寡核苷酸析離(OST)管柱（以1.7µm吸附劑填裝），相較於HPLC、IP-RP LC、, 及快速的LC 解離，可提供優異的寡核苷酸分離之解析效能。
Intelligently designed for optimum performance
The UPLC Oligonucleotide Analysis Solution, based on our award-winning ACQUITY UPLC System separation technology and new OST Columns, provides the ultimate in high-resolution chromatographic performance. And the system offers flexible detection options - such as tunable UV, photodiode array, fluorescence, and mass spectrometry - that are all optimized for UPLC.
Waters considered every aspect of oligonucleotide analysis to create a complete system solution, including:
Expanding the boundaries of resolution and sensitivity
UPLC delivers significant improvements in oligonucleotide analysis when compared to HPLC. Exceptional resolution is possible through the use of UPLC's small particles, which result in dramatically narrower and sharper peaks. Just as significant, the surface chemistry of our second-generation Ethylene-Bridged Hybrid (BEH Technology) OST Columns provides exceptional peak shape for a wide range of oligonucleotide properties.
This remarkable column performance can only be achieved with the optimized instrumentation of the ACQUITY UPLC System, which features reduced system volumes, minimal detector band-broadening, and accelerated data acquisition rates that are required to preserve high-efficiency separations - while maximizing sensitivity.
Designed for reproducibility and reliability
The ACQUITY UPLC System's advanced control of flow rate and precise gradient combines with stable column chemistry to provide highly reproducible chromatography. Long-term assay stability is ensured with columns that are quality-control tested specifically for oligonucleotide analysis.
Qualitative and quantitative analysis of impurities and synthesis artifacts
All elements of the UPLC Oligonucleotide Analysis Solution contribute to the successful qualitative and quantitative analysis of oligonucleotide variants, trace impurities, and truncated sequences. With this combination, oligonucleotides can be detected and measured with the greatest of confidence.
The system's advanced optical and mass detection technologies have a wide dynamic range, ensuring that oligonucleotides and impurities at extreme molar ratios are detected, even at the lowest limits of quantitation.
Oligonucleotide Separation Technology (OST) Columns effectively isolate and analyze DNA, RNA, or modified and chimeric oligonucleotides. In addition, Oasi® HLB solid-phase extraction (SPE) cartridges and plates are ideally suited for sample desalting prior to MS or LC/MS analysis.
OST Columns contain second-generation hybrid-silica BEH Technology particles functionalized with C18. The analysis of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography.
OST chemistry delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of specific application demands
The availability of 1.7-μm UPLC technology and 2.5-μm HPLC particles in a portfolio of optimized column dimensions gives you the flexibility to meet oligonucleotide analysis and laboratory-scale isolation needs.
Highlighted application work:
Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options
The analysis of oligonucleotides by LC coupled with mass spectrometry is becoming a common practice. Many applications require the identification and analysis of oligonucleotides, modifications, variants, and process-related impurities at extremely low concentrations. With versatile configurations that can match your application requirements, Waters' portfolio of MS technologies provides the most appropriate solution for your laboratory's needs.
The ACQUITY SQD combines the resolution, sensitivity, and speed of UPLC technology with single quadrupole MS detection. Designed specifically for chromatographers performing UPLC/MS analysis, it offers a simple yet powerful solution for multi-tasking, fast-paced, or routine laboratory environments - with robust and reliable performance, and walk-up operation for any expertise level.
The ACQUITY UPLC System's ability to produce unmatched resolution, combined with the spectral quality available with the ACQUITY SQD, enables easy identification of compounds in applications, including:
LCT Premier XE
The LCT Premier XE benchtop orthogonal acceleration time-of- flight (oa-TOF) mass spectrometer delivers high sensitivity, resolution, and exact mass measurements for LC/MS oligonucleotide analyses. Its fast data acquisition rates and automated workflow features match the requirements for MS detection under UPLC conditions, including:
The Q-Tof Premier, a quadrupole, orthogonal acceleration time-of- flight (oa-TOF) tandem mass spectrometer, provides enhanced levels of flexibility, sensitivity, specificity, and speed of MS data acquisition. It enables automated exact mass measurement of precursor and fragment ions to yield the highest confidence in structural elucidation and databank search results. Featuring T-Wave (Travelling Wave) Technology, the Q-Tof Premier offers significant enhancements in mass measurement accuracy, dynamic range, sensitivity with Enhanced Duty Cycle, and speed.
Highlighted application work:
UPLC/MS Analysis of Interfering RNA Oligonucleotides
The SYNAPT HDMS and SYNAPT G2 HDMS Systems offer unique, enabling functionality designed for researchers working at the limits of conventional mass spectrometry capabilities who need to further characterize and define their oligonucleotides.
Combining high-efficiency ion mobility-based measurements and separations with quadrupole time-of-flight (TOF) mass spectrometry, the SYNAPT HDMS System enables the analysis of oligonucleotides differentiated by size and shape, as well as mass, to deliver increased specificity and sample definition beyond that achievable by conventional mass spectrometers. This unique capability delivers enhanced sensitivity and mass accuracy, making MS/MS oligonucleotide sequencing possible through the unambiguous assignment of fragment ions
Offering both TOF and HDMS operation modes, the SYNAPT HDMS expands your capabilities beyond conventional MS:
Highlighted application work:
UPLC SYNAPT MS/MS Method for Structural Characterization of siRNA Oligonucleotides