ChromScope Software

ChromScope™ Software is for use on Waters™ supercritical fluid chromatography (SFC) purification systems, supecritical fluid extraction (SFE) systems, and liquid chromatography (LC) for purification. It enables fully automated instrument control, development of SFC methods, and purification of samples. The software also allows manual or automated control of SFE systems and simplifies reporting of conditions.  

Its simple work-flow-based architecture and intuitive user-friendly interface makes routine use easy. Simple wizards guide users through a stepwise process of creating new sequences, including method screening, purification, and calibration curve building.

ChromScope Software can be used with systems configured for extraction, method development, or purification, and is available with:

• SFC 150 Mgm
• Investigator SFC Systems
• SFC 350 Systems
• MV-10 ASFE System (Product has been discontinued)
• Prep SFE Systems (Products have been discontinued)
• BBES (Products have been discontinued)
• Prep 150 LC System

For additional information on ChromScope v2.1 compatability and upgrade requirements, reference our FAQs.

In order to run the instrument, process run results, or collect fractions, methods must be created to define how these operations should be carried out.

Method files fall into three categories:

• Instrument methods: Instrument method files contain information that describes how to operate the devices attached to the system, such as flow, pressure, and temperature settings as well as any device specific settings, the latter being separated onto dedicated tabs in the instrument method editor.

General instrument settings

• Integration methods: Integration method files define the parameters that govern the integration of a chromatogram, and may be applied to any number of chromatograms in the same project.

Integration method

• Collection methods: Collection method files are used to define automated collection parameters.

Collection method

Peaks can be created, deleted, and split using manual integration options. End points of the peaks can be dragged and users can manually set the void time using popup menu of chromatogram plot. Manual integration will automatically update the peak calculations in data grid. Manual peak options in tool bar or menu bar behave like toggle button. Once enabled, options will remain active unless disabled explicitly.

Integration results

The Auto Integration option is used to choose the integration method from the current project and apply it to the data file open in active file tab.

Auto integration settings can be applied:

• To all chromatograms of the file
• Locally, where it is applied only to current chromatogram plot

Supported integration types are:

• Integrate by Threshold
• Integrate by Time
• Integrate by First Derivative
• Integrate by Second Derivative

Supported baseline options are:

• Valley to Valley
• Baseline Drop

ChromScope Software  allows you to create a sequence of injections by selecting methods, setting run times, injection volumes, and various other run parameters. A sequence can be built by either using the Sequence Editor or the Sequence Wizard.

The Sequence Editor allows you to create a new sequence or modify an existing sequence. Manually create/edit sequences using a table format with each run in the sequence being represented by a row in the table, and each option for a run in the sequence being represented by a column.
Sequence Editor

The Sequence Wizard can be used to create method screening sequences, calibration curve building sequences or purification sequences. Sequences created through Sequence Wizard will populate the Sequence Editor.  Users can then save and add it to the sequence queue for execution.

Sequence Wizard

ChromScope Software supports both manual and automatic integration for processing chromatograms allowing you to:

• Set common report layouts for data files to be processed in batch.
• Print batch reports according to the individual integration and report settings of each data file.
• Easily print reports with chromatogram and data grid information to a PDF file or installed printer.
• Select Quick or Custom Reports:
  • Quick Reports have predefined layouts and fields.
  • Custom Reports can create/edit/apply a report template to a date set to define the report layout.
• Build and print a calibration curve based on either height or area.  Supported calibration curve types are; linear, linear through origin, quadratic, and segmented.

Quick and custom reporting formats are available.

Quick Report contains a predefined set of fields, which cannot be altered. Custom Report contains set of components which can be altered using Report Template Editor. The report is printed according to template defined by user.

Main components of the Custom Report include:

• Header
• Plots:
  • Chromatogram - Plot ofdetector signal recorded during the run
  • PDA 3D - Plot of 3 dimensional view of absorbance, time and wavelength
  • Flow – Plot of the CO₂ and co-solvent flow rates during the run
  • Pressure - Plot of the front pressure and back pressure rates during the run
  • PDA Single Spectrum Plot - Plot of wavelength and absorbance
• General Information - General information about the data file
• Injection Information - Injection parameters
• Peak Information - Peak parameters
• Footer

Report template