clinical webinar week - registration page


Waters Clinical Webinar Week: LC-MS for Better Care!


Liquid chromatography-mass spectrometry (LC-MS) is a well-established tool for the identification and quantification of small molecules in research and industrial settings, and has transitioned to a higher-throughput, safer, and lower-cost alternative to immunoassays for routine testing – enabling smaller labs to adopt the technology with ease.

As newer techniques continue to be developed for the clinical laboratory, the analysis of molecules within biological matrices has become more sensitive, specific, and sophisticated. Small molecule analysis can be performed on a variety of sample types in a clinical research setting—including whole blood, serum, plasma, urine and cerebrospinal fluid (CSF).

During Waters Clinical Webinar Week, you will learn how LC-MS can help you in your daily lab routine in various area of clinical diagnostics. Select a topic below or scroll the page to see full agenda, and register to attend our webinars!

 

Clinical Mass Spectrometry: Past, Present and Future
Sensitivity, Selectivity, and Speed: Solving Analytical Challenges in Endocrinology using LC-MS/MS for Clinical Research
Spot the Difference: What Causes Variability in Flow-Injection, Dried Blood Spot Analysis, and What You Can Do About It
IVDR Implementation for Clinical Laboratories
Analysis of Methylmalonic Acid (MMA) in Serum Using the Xevo TQ-S micro for Clinical Research



 



Monday, May 25th - 2pm CET
Clinical Mass Spectrometry: Past, Present and Future

A presentation by Mike Morris - Senior Director of MS research. 

Why attend this webinar?

  • Understand the evolution of clinical mass spectrometry in Waters.
  • Appreciate the versatility of mass spectrometry for addressing clinical problems
  • See what may be possible in the future.
  clinical webinar week - registration page
 

This presentation will focus on the use of soft ionisation techniques applied to problems of clinical interest. The key milestones that Waters has passed in the implementation of mass spectrometry detection into the clinical market will be discussed, and then the early application of electrospray to the study of haemoglobin variants through the application of tandem mass spectrometry to newborn screening and beyond to assays that are in regular use today will be reviewed. The final part of the presentation will look at the potential future uses of technologies in clinical research, as well as considering some of the efforts that are currently underway in supporting the research into Covid-19.

Key literature references will allow the viewer to study the different applications to a greater depth.

NB A basic understanding of ionisation and tandem mass spectrometry is assumed.

 
clinical webinar week - registration page  

Presenter - Mike Morris, Senior Director of MS research - Waters Corporation

Dr Morris received a Ph.D. in mass spectrometry from from UMIST, Manchester in 1988. He has been involved in a number of research projects, including the ion optic design of sector instruments, development of surface-induced dissociation and the application of tandem mass spectrometry. Dr Morris founded the Clinical Operations Group in Waters in 1999, and was responsible for the development of applications of mass spectrometry in neonatal screening, therapeutic drug monitoring and clinical toxicology. In 2012 he was appointed as a visiting Professor in the Department of Surgery and Cancer at Imperial College, London.

 
Register now
 



Tuesday, May 26th - 5pm CET
Sensitivity, Selectivity, and Speed: Solving Analytical Challenges in Endocrinology using LC-MS/MS for Clinical Research

A presentation by Dominic Foley - Senior Scientist

Why attend this webinar?

  • Understand the analytical benefits of using LC-MS/MS for endocrine methods over other platforms
  • Incorporation of selective sample preparation workflows to improve analytical performance
  • Demonstrate how offline automation can be leveraged to improve method performance and optimize efficiency in LC-MS/MS laboratories
  clinical webinar week - registration page
 

The use of LC-MS/MS technologies in Endocrinology for clinical research is experiencing rapid growth. This is a result of the demonstrated improvements in selectivity, and therefore accuracy, provided by the platform compared to more traditional technologies such as immunoassay. In addition, the ability of LC-MS/MS to monitor large number of analytes in a single method, with improvement in both analytical sensitivity and selectivity, increases the speed at which a sample can be interrogated for biochemical abnormalities. Analytical sensitivity, selectivity and speed are all important aspects of endocrine methodologies which can be augmented by using high sensitivity LC-MS/MS platforms such as the Xevo TQ-S micro and Xevo TQ-XS mass spectrometers. For challenging analyses on these platforms, automation can be employed to simplify the workflow to minimize manual intervention during this process, which helps improve both method robustness and laboratory efficiency.

 
clinical webinar week - registration page  

Presenter - Dominic Foley, Senior Scientist - Waters Corporation

Dominic Foley has specialized in the development of LC-MS/MS clinical research applications, with a particular focus on steroid hormone analysis. In his role, he also collaborates with clinical customers in the development and implementation of LC-MS/MS methods in their laboratories. Dominic has worked in separation sciences for 13 years. He has presented his work at conferences around the world and has several publications in both the Microchemical Journal and the Journal of Chromatography A.

 
Register now
 



Wednesday, May 27th - 2pm CET
Spot the Difference: What Causes Variability in Flow-Injection, Dried Blood Spot Analysis, and What You Can Do About It

A presentation by Heather Brown - Principal Scientist

Why attend this webinar?

  • The benefits and limitations of flow injection analysis
  • How instrument and sample factors can contribute to variability between systems
  • How to design a system suitability verification procedure to spot system issues before they affect performance
  clinical webinar week - registration page
 

Tandem mass spectrometry is the detection method of choice when analytical specificity and speed of analysis is needed. Flow-injection analysis tandem mass spectrometry (FIA-MS/MS) enables the rapid and multiplexed screening of compounds, in a single injection. When FIA takes the place of liquid chromatography separations, and we choose internal standardization over multi-point calibration with matrix-based standards, what else should we do to ensure our data is of the very highest quality, as demanded in a clinical setting?

It is important we understand how sample preparation, analytical methods and instrument settings can influence the end result. In having this knowledge, we can address the causes of between- and within-laboratory variation in FIA-MS/MS methods.

This webinar aims to illustrate some of the factors which can influence variability in FIA-MS/MS data, and to provide the audience with ideas for designing system suitability testing procedures to identify sample and instrument-issues before they impact service.

 
clinical webinar week - registration page  

Presenter - Heather Brown, Principal Scientist - Waters Corporation

Heather works in the Scientific Operations group at Waters Corporation, Wilmslow, UK, where she provides technical support to MS/MS laboratories. Before joining Waters Corporation, Heather trained in the United Kingdom National Health Service as a Clinical Scientist. She obtained her doctoral degree from The University of Manchester School of Pharmacy and Pharmaceutical Sciences.

 
Register now
 



Thursday, May 28th - 2pm CET
IVDR Implementation for Clinical Laboratories

A presentation by Marta Carnielli, PharmD, Technical Officer IVD - TÜV SÜD Product Service GmbH

Why attend this webinar?

  • Get an overview of the central changes imposed by the new legislation
  • Understand the role of notified bodies (NBs) under the IVDR
  • Learn how the IVDR affects lab-developed tests (“in-house tests”)
  clinical webinar week - registration page
 

By May 2022, the in-vitro diagnostics regulation (IVDR) will replace the current in-vitro diagnostics directive (IDD) across the EU. The regulations have a broader scope than the previous laws and specifically include software and novel technologies such as genetic testing. In addition, they introduce a new, risk-based classification system for IVDs. Unique device identifiers for certain products and a yet-to-be-established centralized database to collect and share information will be implemented. Manufacturers of commercial products are required to provide strong clinical evidence and establish a post-market surveillance system. Importantly, the percentage of IVD devices requiring notified body approval is going to increase to 90%.

Clinical MS users are affected by delayed publication of IVDR implementation guidelines and notified body certification. Concerns include the continued availability of CE IVD labeled in-vitro diagnostics for rare diseases and the permissibility of lab-developed tests under the IVDR.

Our free webinar will help you understand the implications of the new regulation. Following the webinar, Ms Carnielli will be available for a Q&A.

 
clinical webinar week - registration page  

Presenter - Marta Carnielli, Pharm D, Technical Officer IVD - TÜV SÜD

Marta Carnielli works for TUV SUD as IVD Technical Officer; in this role, she is responsible to gather and distribute regulatory information in the IVD field, represent TÜV SÜD in relevant regulatory committees and conferences and support management and technical experts with the interpretation of regulatory requirements.

Prior to joining TÜV SÜD, Marta worked for Ortho Clinical Diagnostics where she held various roles of increasing responsibility within Customer Technical Support and the Quality, Regulatory and Compliance department. In her most recent role she was Senior Manager, QRC EMEA; in this role she was responsible for quality, regulatory and compliance activities related to the commercial business in Europe, Russia, CIS, Africa and Middle East.

 
Register now
 



Friday, May 29th - 11am CET
Analysis of Methylmalonic Acid in Serum Using the Xevo TQ-S micro for Clinical Research

A presentation by Dominic Foley - Dominic Foley - Senior Scientist

Why attend this webinar? - Key learning objectives

  • Understand the role of Vitamin B12 and its impact on methylmalonic acid concentrations
  • Utilizing selective chromatographic separation to providing precise and accurate data for MMA
  • Demonstrating how a simple in-well precipitation can provide an easy to use, high throughput workflow
  clinical webinar week - registration page
 

Vitamin B12 plays an integral role in critical biological functions, such as DNA synthesis, formation of erythrocytes, and maintenance of the myelin sheath in the nervous system. In its role as a co-factor, vitamin B12 aids the methyl-malonyl-CoA mutase catalyzed conversion of methylmalonyl-CoA to succinyl-CoA, with methylmalonic acid (MMA) being an important by-product in this pathway. MMA concentrations are elevated when there is insufficient vitamin B12 to help catalyze this reaction, meaning that MMA is a candidate biomarker for clinical research investigations into the function of vitamin B12. LC-MS/MS is an ideal platform to perform this analysis. Chromatographic separation is key in separating MMA from isobaric and isomeric species, such as succinic acid, that can cause a significant concentration bias. To help facilitate adoption of this methodology, a simplified workflow is necessary to enable a high throughput of samples.

Here we describe a clinical research method utilizing Ostro phospholipid removal plate technology for the extraction of MMA from serum. Chromatographic separation was performed on an ACQUITY UPLC I-Class System using an ACQUITY UPLC 1.7 μm CSH C 18 Column followed by detection on a Waters™ Xevo TQ-S micro Tandem Quadrupole Mass Spectrometer to enable quantification of very low physiological concentrations of MMA.

 
clinical webinar week - registration page  

Presenter - Dominic Foley, Senior Scientist - Waters Corporation

Dominic Foley has specialized in the development of LC-MS/MS clinical research applications, with a particular focus on steroid hormone analysis. In his role, he also collaborates with clinical customers in the development and implementation of LC-MS/MS methods in their laboratories. Dominic has worked in separation sciences for 13 years. He has presented his work at conferences around the world and has several publications in both the Microchemical Journal and the Journal of Chromatography A.

 
Register now