Practical Approaches to Peptide Isolation [715005429]

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Practical Approaches to Peptide Isolation As the interest in peptides as potential drug candidates continues to grow the need for basic information about the isolation and purification of these peptides also grows. Whether peptides are made step-wise using solid-phase peptide synthesis, solution phase synthesis, or isolated from naturally-occurring sources, almost all crude peptide mixtures are complex and require purification. The Practical Approaches to Peptide Isolation, a 84 page book, discusses the peptide isolation workflow, method development considerations including column selection, choice of mobile-phase modifier, the use of temperature, and gradient optimization, along with other relevant topics. The use of mass-directed isolation which makes the purification process easier with less ambiguous discrimination between the target peptide and the contaminants is also discussed.

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