Literature


Description
Development of a Sensitive High Resolution Mass Spectrometry Method for Quantitation of N Nitrosamin...
Library Number: APNT135071818 | Format: PDF | File Size: 1068.72 kB | Year: 2020 | Content Type: Application Notes

This application note demonstrates the ability to attain both sensitive and selective detection of six N-nitrosamines using Tof-MRM acquisition on the Xevo G2-XS QTof.

Comprehending COVID-19: Fast Analysis of Ribavirin and Related Compounds Using Hydrophilic Interacti...
Library Number: APNT135071822 | Format: PDF | File Size: 521.06 kB | Year: 2020 | Content Type: Application Notes

This application note reports a method for the separation of ribavirin and related compounds using HILIC.

Size Exclusion Chromatography Method Transfer from a Standard HPLC to an Arc HPLC System
Library Number: APNT135071867 | Format: PDF | File Size: 609.01 kB | Year: 2020 | Content Type: Application Notes

This application brief demonstrates a size-exclusion chromatography (SEC) method transfer for monoclonal antibody (mAb) analysis from an industry-standard HPLC system to a Waters Arc HPLC System.

Lessons from COVID-19: Verifying Performance of an HPLC/UV method with MS Compatible Conditions for ...
Library Number: APNT135071733 | Format: PDF | File Size: 782.6 kB | Year: 2020 | Content Type: Application Notes

In this application brief we present a fast and reliable HPLC/UV method for hydroxychloroquine sulfate analysis with use of MS compatible buffer, while meeting the USP system suitability requirements.

MetaboQuan-R for the UPLC-MS/MS Analysis of Steroids
Library Number: APNT135071748 | Format: PDF | File Size: 724.83 kB | Year: 2020 | Content Type: Application Notes

Here we demonstrate a high-throughput UPLC-MS/MS research method for the semi-quantitative analysis of 14 steroids in human plasma/serum samples, without the need for derivatization, and using a generic, flexible LC-MS platform that has previously been us...

Best Practices for Oligonucleotide Analysis Using Ion-Pair Reversed-Phase (IP-RP) Liquid Chromatogra...
Library Number: APNT135071755 | Format: PDF | File Size: 1177.92 kB | Year: 2020 | Content Type: Application Notes

In this application brief, Waters provides a short list of best practices to characterize oligonucleotides by ion-pair reversed-phase (IP-RP) liquid chromatography. These best practices cover separation temperature, pH optimization, pore size selection, m...

A Robust and Reproducible Reversed-Phase Lipid Profiling Method for Large Sample Sets
Library Number: APNT135071759 | Format: PDF | File Size: 842.93 kB | Year: 2020 | Content Type: Application Notes

In this work we present a high throughput, robust, and reproducible LC-MS methodology RP-UPLC lipid separation using a Waters ACQUITY UPLC I-Class PLUS System with Charged Surface Hybrid (CSH) C18 chemistry and QTof detection. The combination of sub–2–µm ...

High-Throughput Amino Acid Analysis Using Hamilton Automated Preparation
Library Number: APNT135071764 | Format: PDF | File Size: 1052.96 kB | Year: 2020 | Content Type: Application Notes

The objective of this application note is to demonstrate the equivalency and robustness of manual preparations of AccQ●Tag-labelled amino acids to those prepared using a Hamilton Microlab (ML) STAR automation platform using the Amino Acid Cell Culture Sta...

High-Throughput Amino Acid Analysis using Tecan Automated Preparation
Library Number: APNT135071769 | Format: PDF | File Size: 1097.24 kB | Year: 2020 | Content Type: Application Notes

In this application note, we will demonstrate the equivalency of manual preparations of AccQ●Tag labelled amino acids to those prepared using a Tecan Freedom EVO 100/4 automation platform with an Amino Acid Cell Culture Standard Kit.

Assessing the Chromatographic Performance of Small Polar Compounds when Using HILIC-based LC-MS Chro...
Library Number: APNT135071776 | Format: PDF | File Size: 738.72 kB | Year: 2020 | Content Type: Application Notes

This application brief assess the performance and robustness of HILIC and BEH Amide Column chemistries for the separation of hydrophilic polar metabolites and their suitability for metabolic profiling studies.

Effective Use of Survey Scan on Xevo TQ-XS for Qualitative Analysis of Herbal Samples
Library Number: APNT135071782 | Format: PDF | File Size: 1452.62 kB | Year: 2020 | Content Type: Application Notes

This application brief demonstrates to increase the efficiency and productivity for qualitative analysis time of complex samples like herbal extracts.

Comprehending COVID-19: Rapid and Sensitive Characterization of N-Glycans from SARS-CoV-2 Spike Prot...
Library Number: APNT135071689 | Format: PDF | File Size: 606.02 kB | Year: 2020 | Content Type: Application Notes

This work utilizes the GlycoWorks RapiFluor-MS N-Glycan Kit to easily and rapidly detect SARS-CoV-2 spike protein N-Glycans.

Comprehending COVID-19: Preliminary Examination of the SARS-CoV-2 Spike Protein by Peptide Mapping
Library Number: APNT135071693 | Format: PDF | File Size: 744.32 kB | Year: 2020 | Content Type: Application Notes

In this app brief, we apply a tryptic peptide mapping approach to analyze a recombinant SARS-CoV-2 spike protein using a BioAccord LC-MS system.

Rapid Identification of Major Metabolites of Gefitinib Using Ion Mobility Enabled MS (HDMSE) with th...
Library Number: APNT135071695 | Format: PDF | File Size: 523.73 kB | Year: 2020 | Content Type: Application Notes

This application note demonstrates the use of the SYNAPT XS combined with the Waters UNIFI Metabolite Identification Application Solution for identification of the drug-related metabolites of gefitinib (Iressa) in mouse plasma.

Comprehending COVID-19: Reversed-Phase Liquid Chromatography (RPLC) of Intact SARS-CoV-2 Spike Prote...
Library Number: APNT135071700 | Format: Web Page | File Size: - | Year: 2020 | Content Type: Application Notes

This application brief focuses on reversed-phase liquid chromatographic analysis of the intact SARS-CoV-2 spike protein which has emerged as a potential target for vaccine development due to its role in viral pathogenesis.

Ion Mobility for Metabolite Characterization: Improving Spectral Clarity of Data Independent Acquisi...
Library Number: APNT135071703 | Format: PDF | File Size: 291.98 kB | Year: 2020 | Content Type: Application Notes

This application brief demonstrates that spectral alignment applying drift time alongside retention time can significantly improve spectral clarity aiding structural characterization for the interpretation of metabolites/degradants of dextromethorphan inc...

Lessons from COVID-19: Analysis of Azithromycin using Hydrophilic Interaction Chromatography with LC...
Library Number: APNT135071687 | Format: PDF | File Size: 191.66 kB | Year: 2020 | Content Type: Application Notes

This app brief describes various analytical methods for bioanalysis applications of azithromycin.

Arc HPLC System: Improved Productivity and Seamless Transfer of an HPLC Impurities Method
Library Number: APNT135071584 | Format: PDF | File Size: 1237.81 kB | Year: 2020 | Content Type: Application Notes

This application brief demonstrates seamless transfer of an HPLC impurities method to an Arc HPLC System and to a smaller particle column for improved laboratory efficiency and productivity.

How-to-Series Video 3: Application Note
Library Number: APNT135071436 | Format: PDF | File Size: 2717.17 kB | Year: 2020 | Content Type: Application Notes

How To Video Episode #3 – Assessing and compensating for matrix effects will help the sensitivity, accuracy and robustness of your analytical method

How-to-Series Video 2: Application Notes
Library Number: APNT135069380 | Format: Web Page | File Size: - | Year: 2020 | Content Type: Application Notes

How-to-Series Video 2 - Getting sample preparation right is probably the most important step in any analysis

High Sensitivity Quantitation of Nitrosamine Genotoxic Impurities: LC-MS Analysis of Ranitidine Drug...
Library Number: APNT135068607 | Format: PDF | File Size: 1213.39 kB | Year: 2020 | Content Type: Application Notes

This application note presented herein, provides practical considerations for optimization of LC-MS conditions to achieve sensitive and robust simultaneous quantification of several nitrosamine GTIs (NDMA, NDEA, NEIPA, NDIPA, NDBA, and NMBA).

Size-Exclusion Chromatography (SEC) of Proteins and Peptides Application Notebook
Library Number: APNT135068236 | Format: PDF | File Size: 34443.85 kB | Year: 2020 | Content Type: Application Notes

In this application notebook, Waters has compiled the following references to help guide through some common challenges and applications associated with SEC of peptides and proteins. The topics covered range from fundamentals of SEC to suggested best prac...

Quality Control and Automation Friendly GlycoWorks RapiFluor-MS N-Glycan Sample Preparation
Library Number: APNT134865160 | Format: PDF | File Size: 779.27 kB | Year: 2015 | Content Type: Application Notes

This application note demonstrates RapiFluor-MS glycan labeling procedure with larger volume, simplified liquid transfer to improve ease of use and automatability.

Waters Food Testing Application Notes Booklet
Library Number: APNT134800757 | Format: PDF | File Size: 11822.48 kB | Year: 2020 | Content Type: Application Notes

Read our in-depth application notes to learn about the latest analytical methods for pesticides, food allergens, veterinary drugs, food contact materials, natural toxins, food authenticity, and food ingredients testing.

Application of Intentionally Attenuated MRM Transitions for the Analysis of Natural Products
Library Number: APNT135067849 | Format: PDF | File Size: 820.91 kB | Year: 2020 | Content Type: Application Notes

This application brief demonstrates to develop intentionally attenuated MRM transitions in order to allow the quantitative analysis of natural product constituents of disparate abundance within a single acquisition.