Repeatability of the ACQUITY UPLC H-Class PLUS Bio Binary System Under Challenging Separation Conditions

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David Dao, Brooke M. Koshel, Robert E. Birdsall, Ying Qing Yu
Waters Corporation
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Peptide mapping analyses often employ low flow rates and shallow gradients, conditions that are considered challenging for developing robust analytical methods. Although some variability in retention time is expected under these conditions, retention time repeatability is critical for proper peptide identification and quantitation. This work demonstrates that the ACQUITY UPLC H-Class PLUS Bio Binary System can be used to deliver exceptional gradient precision and to minimize the retention time variability experienced under challenging method conditions.

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