Method Migration of Size-Exclusion and Ion-Exchange Methods for Monoclonal Antibody Analysis from HPLC to the Arc Premier System

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Brooke M. Koshel, David Dao, Robert E. Birdsall, Ying Qing Yu
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Traditional SEC and IEX methods employ HPLC systems and column chemistries having large particle size to avoid exceeding pressure limitations of these more aged platforms. As more modern LC platforms are incorporated into the laboratory, these new systems must demonstrate that they can produce comparable results to existing technologies. This work demonstrates that legacy SEC and IEX methods could be migrated from an Alliance HPLC System to an Arc Premier System with negligible differences in retention time and peak area percent. By combining the Arc Premier System with modern UHPLC columns, data could be more accurately interpreted through greater efficiency, selectivity, and enhanced resolution.

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