SPE Considerations for UPLC-MS Determination of Perfluorinated Carboxylic Acids and Perfluoroalkylsulfonates in Water and Tissue

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Michael S. Young;Kimvan Tran [Waters]
226th ACS Fall Meeting , September 7-11, New York City; NY
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Recently, it has become evident that perfluorinated compounds (PFCs) such as perfluorooctanesulfonate and perfluorooctanoic acid are persistant organic pollutants (POPs) and are ubiquitous in the environment (Smithwick et al., 2005). Because PFCs may be toxic and have bioaccumulative properties, there is growing interest in the development of analytical methods for PFCs in food, drinking water, tissue, plasma and blood. In this presentation we will discuss sample preparation issues and instrumental requirements for UPLC-MS determination of PFCs in water and tissue samples. Among the topics will be the choice of solvent extraction and solid-phase extraction (SPE) methods used in sample preparation and the optimization of LC-MS for this analysis. For example, the quantitation limit for perfluorooctanesufonate (PFOS) is well under 0.1 µg/L for a 100 mL water sample processed using a mixed-mode weak anion-exchange SPE cartridge and analyzed using UPLC-MS in negative electrospay mode. Quantitation limits of 1 µg/L were achieved for most PFCs in tissue samples such as chicken liver and muscle.

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