Development of a Rapid andSensitive LC-MS/MS Method for The Simultaneous Identification and Quantitation of Corticosteroids in Bovine Liver Extracts

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Hamoir, T[1];Pottie, G[1];Degroodt, J-M[1];Hewitt, C[2];Van eeckhout, N[3];de craene, B[3];Clauwaert, K[3];Claereboudt, J[3].
4th Int. Symposium on Harmone and Veterinary Drug Residue Analysis; Antwerp; 4th-7th June; 2002
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Corticosteroids are used in many veterinary therapeutic drugs for their anti-inflammatory properites. However, they are also illegally used as growth promoters in cattle because these compounds increase the gain in weight of some animals and have a synergethic effect with other growth promoters To control the use of these growth promoters a rapid and sensitive method was developed for the analysis of 6 corticosteroids (Triamcinolone Acetonide, Flumethasone, Dexamethasone, Betamethasone, Methylprednisolone and Prednisolone) in bovine liver extracts using liquid chromatography tandem mass spectrometry. Dexamethasone-d[3] was used as an internal standard. Considering European Union legislation, special interest was shown into the separate confirmation and quantification of dexamethasone and betamethasone residues in liver extracts. This study was based on an earlier study performed by the laboratory of Prof. C. Van Peteghem[1]/ However in this report, compared to the previous study, a wider range of compounds (total of 6 different corticosteroids) was analysed on a higher end instrument (Quattro Ultima).

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