Study of Bonded Phases Cleaved from Silica-Based Liquid Chromatographic Packing Materials by Electrospray Ionization-Mass Spectrometry
Liquid chromatography/electrospray ionization-mass spectrometry
(LC/ESI-MS) has become an important tool in proteomics, genomics, and
drug metabolism and pharmacokinetics. However, it has been noticed that
bleeding from liquid chromatographic packing materials may interfere
with the characterization of analytes and reduce detection sensitivity.
The bleeding substances can be cleaved bonded phases and residual
reagents from silica-based materials, or degradation products, residual
reagents and small oligomers from polymeric materials. Although ESI-MS
has been used to analyze metal ions, inorganic and organic acids, and
organic bases, few reports have been found on the characterization of
organic silanols, the hydrolysis products of organic silyl ligands
bonded on silica particles. In our work, we have focused on the
understanding of the behavior of bonded phases cleaved from the
silica-based materials under acidic and basic conditions.