Study of Bonded Phases Cleaved from Silica-Based Liquid Chromatographic Packing Materials by Electrospray Ionization-Mass Spectrometry

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Yuehong Xu, Tom Walter [Waters]
ASMS 2003
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Liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS) has become an important tool in proteomics, genomics, and drug metabolism and pharmacokinetics. However, it has been noticed that bleeding from liquid chromatographic packing materials may interfere with the characterization of analytes and reduce detection sensitivity. The bleeding substances can be cleaved bonded phases and residual reagents from silica-based materials, or degradation products, residual reagents and small oligomers from polymeric materials. Although ESI-MS has been used to analyze metal ions, inorganic and organic acids, and organic bases, few reports have been found on the characterization of organic silanols, the hydrolysis products of organic silyl ligands bonded on silica particles. In our work, we have focused on the understanding of the behavior of bonded phases cleaved from the silica-based materials under acidic and basic conditions.

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