Study of Loadability and Selectivity of Pharmaceutical Compounds on RPLC Columns

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Fang Xia, Jie Y. Cavanaugh, Uwe Neue, Jeffrey R. Mazzeo, Diane M. Diehl [Waters]
AAPS, Salt Lake City Utah, October 26-30
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Nordoxepin; Doxepin; Amitriptyline; Cinoxacin; Oxolinic acid; Nalidixic acid; Pyridoxal; Folic acid; Caffeine; Indoprofen; Naproxen; Flurbiprofen; Hydrocortisone; Medroxyprogesterone 17-acetate; Medroxyprogesterone; Prednisolone; Dexamethasone; Betamethasone 17-valerate; Progesterone; Catechins; Epicatechin; Epigallocatechin Conditions:
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One of the challenges that the preparative chromatographer has is to maximize column loading to keep the costs of operation and equipment as low as possible. Therefore, it is very necessary to study the factors that enhance column loading. In this presentation, we studied the loading of 30 compounds on 12 RPLC columns with large varieties in stationary phase chemistry and ligand densities. The test compounds were selected based on their structural characteristics and classified into several groups. Mobile phase pH effects on column loadability and selectivity were studied. In addition, the effects of additives on column loadability were compared as well. The preliminary results demonstrate that the particle chemistry, ligand type, nature of the compound, pH and additives in mobile phases all have significant contributions to the column loadability and selectivity.

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