Rapid, Selective Screening of Urine Samples for Glucuronides by LC/MS/MS

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Waters Poster
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Other Symposium
The traditional study of in vivo drug metabolism in plasma or urine samples is often complicated by the presence of many endogenous compounds. Several mass spectrometric techniques are often applied in drug metabolism studies. Conjugation reactions, particularly glucuronidation, can be studied using either the neutral loss or precursor ion experiment, however the experiments are not usually done at the same time. Using a hybrid quadrupole/time-of-flight instrument, a new method of performing the neutral loss and precursor ion scan experiments simultaneously hav been devised. Using this novel approach, which is more sensitive and selective than the corresponding triple quadrupole experiments, several Phase 1 and Phase 2 metabolites of diphenhydramine were identified from a human urine sample collected 4 hours after drug administration.

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