A Novel Strategy for the Analysis of Phosphopeptides, Coupling N-terminal Peptide Derivatization and HPLC Separation with Mass Spectrometry

Library Number:
720001107EN
Part Number:
720001107EN
Author(s):
J. I. Langridge1, J. Rontree2, E. Claude1, I. Campuzano1, M. Snel1, T. McKenna1.1Waters Corporation, Manchester, United Kingdom. 2Waters NV, Almere, The Netherlands
Source:
Presented at: ABRF 2005; Association of Biomolecular Resource Facilities Annual Meeting; February 5-8; Savannah GA
Content Type:
Posters
Content Subtype:
ABRF
Related Products:
Q-Tof Premier
This poster details a preliminary evaluation of N-terminal derivatization, using TMPP-Ac-Osu, for the enhanced analysis of phosphopeptides We have studied the effect of TMPP derivatization on the HPLC performance for small hydrophilic phosphopeptides This approach has been coupled with nanoscale HPLC on-line to an ESI Q-Tof Premier™ In addition off-line HPLC coupled with MS/MS on a Q-Tof Ultima™ MALDI and PSD MX™ on a MALDI micro MX™ has been used.

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