A comprehensive comparison of the selectivity, peak shape, and stability of C18, C8, and phenyl ligands bonded to the same base-silica
With over 600 commercially available C18 packing materials on
the market, the introduction of a new brand of C18 stationary phase
makes choosing a column even more difficult. However, each new brand of
C18 stationary phase often includes additional ligand chemistries that
can be explored for optimal methods development. The performance
attributes of chemistries such as C8 and phenyl stationary phases are
not as well discussed as C18 stationary phases in the literature. The
relative merits of each of these different phases can be exploited in
the development of more robust methods.
The recent introduction of the SunFire™ brand of reversed-phase
packing materials permits a systematic study of the selectivity, peak
shape, and high- and low-pH stability of the various phases in the
family: C18, C8, and phenyl. Because these ligands are bonded to the
same base-silica particle, the observed differences can be attributed
to differences in the surface modification.