1.0 mm i.d. Columns Packed with 1.7 µm Particles Improve LC/ESI-MS Limits of Detection

Library Number:
WA40496
Part Number:
WA40496
Author(s):
Eric S. Grumbach, Diane M. Diehl and Jeffrey R. Mazzeo [Waters]
Source:
ASMS
Content Type:
Posters
Content Subtype:
ASMS
Compounds:
Verapamil; Norverapamil; Methoxyverapamil
Related Products:
Many LC/ESI-MS assays use 2 mm i.d. columns packed with 5 µm particles. This situation is a result of the widespread availability of these columns, the compatibility of the optimal flow rates of these columns with electrospray ionization interfaces, and the ability of modern HPLC instrumentation to run these columns with minimal loss in performance due to extra-column bandspreading. Utilizing 1.7 µm particles and 1 mm i.d. columns should lead to an improvement in sensitivity leading to lower limits of detection. These improvements are dependant on having an LC system that operates at higher pressures and has minimal system volume.

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