Can Ion Suppression be Quantitated?
LC/MS/MS is without question the preferred platform for the
analysis of organic molecules from complex matrices. Most applications
utilize electrospray ionization (ESI) interface (about 80 % of
published papers). ESI is prone to a phenomenon called ion
suppression. It can lead to adverse effects during quantitation (i.e.
poor accuracy and precision). Little has been done to effectively
quantitate which parameters of an extraction or analytical protocol
will cause ion suppression or enhancement. Previous papers have
reported that ion suppression is a direct result of endogenous material
present in biological samples. The model does not show exactly where
and how much suppression can be expected from each analytical step used
in the preparation and analysis of those samples.