Can Ion Suppression be Quantitated?

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Claude R. Mallet;Ziling Lu;Jeff R. Mazzeo [Waters]
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Propranolol ; Trimethoprim; Pipenzolate; Resperidone; Terfenadine; Methoxy-verapamil; Benextramine; Reserpine; Fumaric Acid; Malic acid; Etidronic Acid; Clodronic acid; Niflumic acid; Canrenoic acid; Cholic acid; Raffinose
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LC/MS/MS is without question the preferred platform for the analysis of organic molecules from complex matrices. Most applications utilize electrospray ionization (ESI) interface (about 80 % of published papers). ESI is prone to a phenomenon called “ion suppression.” It can lead to adverse effects during quantitation (i.e. poor accuracy and precision). Little has been done to effectively quantitate which parameters of an extraction or analytical protocol will cause ion suppression or enhancement. Previous papers have reported that ion suppression is a direct result of endogenous material present in biological samples. The model does not show exactly where and how much suppression can be expected from each analytical step used in the preparation and analysis of those samples.

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