Evaluation of Charge State Separation Using a Novel Travelling Wave Based Ion Mobility Device Coupled with an OA-TOF

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Kevin Giles, Jason L. Wildgoose, Steven D. Pringle, Robert H. Bateman,
Waters ASMS 2004 poster
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The improvement in instrumental sensitivity specifically in the field of proteomics is a major driving force for manufacturers. However, quite often the doubly charged peptide ions of interest for MS/MS analysis from a protein digest can be present at analytically useful levels but are swamped by singly charged background, preventing their detection. The utility of mobility separation of ions with similar m/z values but different charge states to enhance detection limits has been shown to be effective when used in a linked scan mode with a quadrupole operating in RF-only mode [1]. In this study it was shown that the observed systematic difference in drift times for ions of different charge state can be used to effect a low-mass cut-off for removal of singly charged species. This is shown schematically in Figure 1. Presented here are the investigations into the charge state separation capabilities of a novel travelling wave based ion mobility separator in conjunction with an oa-ToF.

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