Simplified Protein Precipitation: A New Technology for Precipitation and Filtration

Library Number:
WA40507
Part Number:
WA40507
Author(s):
Kimvan Tran1, Diane M. Diehl1, Ziling Lu1, Tim Schultz2, Wilf Wixwat2, Brian P. Murphy1, Jeff Kane2, Emily Berlin2, Kevin Seeley2, Jeffrey R. Mazzeo1 [1Waters Corporation, Milford, MA];[2Pall Life Sciences, Ann Arbor, MI]
Source:
HPLC
Content Type:
Posters
Content Subtype:
HPLC
Related Products:
To address performance issues on commercially available protein precipitation plates, we developed a new 96-well protein precipitation and filtration plate that offers a dripless technology with a gradient of purpose-selected filtration media, together with a vented closure to allow for rapid ‘in-well’ sample preparation. We ran a series of ion suppression and extraction studies to ensure the cleanliness of the plate materials. We validated the plate performance for a variety of polar and non-polar acids, bases and neutrals spiked into porcine and rat plasmas. We compared the results to traditional centrifugation methods and other commercially available products. In our studies, we did not observe liquid flow until vacuum was applied. Additionally, less than 1% of the wells plugged — and in most plates we did not see any wells plug. The time for filtration ranges from 30 seconds to 3 minutes, depending on the type and amount of plasma, and the time to process an entire plate of 96 samples is approximately 15 minutes. There is no observable cross talk between the wells. The eluents are free of observable particulates and offer a cleaner eluent than the traditional centrifugation method. This new Sirocco™ Protein Precipitation Plate out-performs current commercially available products.

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