Synthesis and Characterization of Hybrid Organic/Inorganic Particles Containing Organo-Reactive Groups for Reversed-Phase HPLC

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Julia Ding, Alan Pelissey, Dan Walsh, Jim Cook, John O''Gara
HPLC 2002; Montreal; 5-6 June 2002
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In reversed-phase HPLC separations, hybrid organic-inorganic particles have been shown to give improved peak shape for bases and exceptional stability in alkaline (pH 12) mobile phases. To date, first generation hybrid particles have consisted of inorganic SiO2 units and essentially inert organic units, such as the methyl group (-CH3). In the present report, we will introduce a new generation of hybrid particles, having organo-reactive groups incorporated throughout the particles. These organo-reactive groups, such as propanol and vinyl groups, are directly derivatized using non-silane chemistry. In comparison to conventional bonded silica particles, we will show how these novel HPLC stationary phases can offer excellent combined particle and bonded phase stability. These novel bonded particles also give a much lower LC-MS bleed than conventional bonded phases made through silane chemistry. In the future, organo-reactive surface bonding may lead to particles with novel selectivity in comparison to silica bonded with classical silane chemistry.

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