Speeding metabolic stability assays using automated high throughput LC/MS techniques
In drug discovery, metabolic stability is often a key factor in
whether or not a compound continues on in the development process.
Metabolic stability can be assessed in vitro using pooled liver
microsomes obtained from humans or other species of interest. To meet
the growing demand for rapid analysis of the large number of samples
generated by metabolic stability assays, automation has become
necessary to increase sample analysis. The use of LC/MS analytical
methods provides the required selectivity, sensitivity, and speed to
produce quality data. This presentation will demonstrate the use of
LC/MS to evaluate samples produced in a metabolic stability assay.
Taking advantage of such factors as parallel sample processing, high
sample capacity, alternating column regeneration, and smaller diameter
columns to reduce cycle times, we can analyze the large number of
samples produced, and ultimately help expedite the drug discovery