Chromatographic Evaluation of a New Organic/Inorganic Hybrid Reversed-Phase HPLC Packing

Library Number:
B Alden;C Gendreau;P Iraneta;T Walter
HPLC''99; Granada Spain; 31 May 1999
Content Type:
Content Subtype:
Nortriptyline; Salicylic Acid; Ibuprofen; Benzoic Acid; Amphetamine; Methamphetamine; Nortriptyline; Nicotine; Propranolol
XTerra RP18; & RP8 MS C18 & C8 5 µm Steel 4.6 mm 150 mm
An organic/inorganic hybrid porous particle has been synthesized for use as an HPLC packing. This new chromatographic hybrid particle has been additionally surface bonded to attach multiple C8 and C18 groups. These surface bonded hybrid particles not only exhibit extended pH stability, but show improved performance for basic compounds. On silica-based reversed-phase columns, broad and tailing peaks for many basic analytes have been attributed to surface silanols. We attribute the improved performance of the surface bonded hybrid particles to their reduced surface silanol concentration. Peak shape for a variety of basic, neutral, and acidic analytes was studied on reversed-phase bonded hybrid packings. Data is presented illustrating peak shape trends as a function of injected mass, mobile phase pH, and organic modifier.

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