Improved Yield and Productivity of Ionizable Compounds Employing Hybrid Packings.
Sample loading onto a column can be highly compromised due to
the solubility of the compounds or their compatibility with the initial
mobile phase conditions, resulting in low yields and productivity.
Consequently, exploring alternative ways to load sample onto the column
is urgently needed. The at-column-dilution method is a significant
alternative as it allows an increase in sample loading, improving peak
shapes, providing higher yield and productivity of the targeted
compound. Case studies are shown where the benefits of the
at-column-dilution method are demonstrated when loading basic compounds
under acidic conditions and eluting at high pH, as well as when loading
samples dissolved in strong sample solvents such as DMSO. The cases
shown here illustrate the utility of the at-column-dilution technique
while maintaining present day isolation and purification needs both in
combinatorial chemistry as well as drug development.