A Method For Detecting Post Translational Modifications In Intact Proteins and Peptides.
Characterization of pharmaceutical proteins and peptides is an
important analytical challenge that must be met in pharmaceutical and
biotechnology companies today. Small chemical variations in proteins
and peptides can be the difference between an active and an inactive
therapeutic. In this study, we looked as some of the more common
modifications; carbamylation, oxidation, formylation, deamidation, and
acylation that are a byproduct of production, purification, and/or
formulation. We have developed a comprehensive and rapid LC/MS
methodology that can indicate the presence and degree of
posttranslational modification using intact proteins or peptides. Our
methodology is based on reversed-phase chromatography coupled with mass
spectrometry since LC-MS is becoming a choice for the determination of
post translation modifications.