Identification and Characterization of Oxidation and Deamidation of Proteins and Peptides by LC/MS(/MS)
We Have developed a simple and rapid LC/MS method for
identifying the presence and degree of oxidation and deamidation of
intact proteins or peptides in a mixed sampling using either single
quadrupole or Q-TOF type mass spectral instrumentation.
Characterization of proteins is an important analytical challenge that
must be met in pharmaceutical and biotechnology companies today.
Reversed-phase chromatography coupled with mass spectrometry has
rapidly become the technique of choice for the determination of