Identification and Characterization of Oxidation and Deamidation of Proteins and Peptides by LC/MS(/MS)

Library Number:
WA20716
Part Number:
WA20716
Author(s):
Paul Rainville;Jeff Mazzeo;Uwe D. Neue;Reb Russell II [Waters]
Source:
Fall ACS 2002; 224th ACS National Meeting; Boston August 18-22
Content Type:
Posters
Content Subtype:
ACS
Compounds:
Protein; Peptide; Alpha Caesin; RNAse
Column:
Symmetry300(™) C18 300 Å 5 µm Steel 4.6 mm 50 mm Atlantis dC18 3 µm Steel 4.6 mm 50 mm
Related Products:
 
We Have developed a simple and rapid LC/MS method for identifying the presence and degree of oxidation and deamidation of intact proteins or peptides in a mixed sampling using either single quadrupole or Q-TOF type mass spectral instrumentation. Characterization of proteins is an important analytical challenge that must be met in pharmaceutical and biotechnology companies today. Reversed-phase chromatography coupled with mass spectrometry has rapidly become the technique of choice for the determination of post-translational modifications.

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