Preliminary Investigations of a Diuretic Screening Method Converted from HPLC/MS/MS to UPLC/MS/MS
The World Anti-Doping Agency (WADA) states all laboratories
must be able to confidently detect the minimal required performance
limit (MRPL) of 250 ng/mL. The increased number of athlete samples
being tested each year has led to a need for a high throughput rapid
screen for prohibited substances. Here we describe a rapid and
sensitive UPLC/MS/MS method for the screening of diuretic compounds.
The conversion of a qualitative diuretic screening method used in
sports doping analysis from HPLC/MS/MS (total run time of 10 min.) to
UPLC/MS/MS, where the analysis time is reduced without any loss of
sensitivity and selectivity. This poster was presented at The
International Association of Forensic Toxicologists (TIAFT), Aug.
26-30, 2006 in Ljubljiana, Slovenia.