CID for the Identification of Drug Metabolites using UPLC/oa-TOF Technology

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Lisa J Calton, Michael McCullagh, Jose Castro-Perez, Hilary Major, Steve Preece
IMSC 2006, Aug. 27-Sept. 1, Prague
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Other Symposium
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Here we present data using high resolution liquid chromatography coupled with collision induced dissociation (CID) and exact mass measurement with oa-TOF for metabolism studies. Combining the chromatographic resolution of UPLC with pseudo MS/MS (CID) and exact mass to determine actual elemental compositions, provides greater confidence in metabolite confirmation. Accurate mass fragment information from CID allows structural information to be derived for the metabolites. When using CID rather than traditional MS/MS, the enhanced resolution of UPLC helps to reduce co-elution and produce clean fragment spectra for easier datainterpretation. When using this approach a routine and rugged hardware configuration is required. This poster was presented at the 17th International Mass Spectrometry Conference (IMSC), Aug. 27-Sept. 1, 2006 in Prague.

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