The enhanced chromatographic resolution associated with Ultra Performance Liquid Chromatography™ has been demonstrated for peptide mapping. The technique improves resolution by a factor of three or more. The Peptide Separation Technology Columns for UPLC™ include both 130Å and 300Å pore size materials. Coupling UPLC™ to oa-Tof mass spectrometry allows high sensitivity identification of glycopeptides through exact mass measurement and deconvolution of complex mass spectra. Optimized chromatographic resolution of individual glycoforms of glycopeptides yields high quality mass spectra. This spectral information can be used to develop UPLC™ methods for quantitative glycopeptide mapping with UV detection.