A Comparison of HPLC and UPLC for Metabolite Screening in Drug Discovery Using TOF-MS
Ultra Performance Liquid Chromatography system (UPLC) and HPLC
are compared to show the difference between the two technologies when
coupled with a TOF-MS and triple quadrupole mass spectrometer for
metabolism studies. In order to show the potential of this particular
approach, a number of analytical runs will be shown, where different in
vitro incubations are compared for Buspirone and Midazolam.