Purification of Ginseng Extract Using Reversed-Phase Liquid Chromatography

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Jie Y. Cavanaugh, Fang Xia, Diane M. Diehl and Jeffrey R. Mazzeo [Waters]
HPLC 2006, June 17-23, San Francisco, CA
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Ginseng has a long history of medicinal use in Asia. The active ingredients from ginseng, ginsenosides, are reported to reduce mental impairment and cell loss from brain degeneration. Many researchers are interested in isolating these active ingredients from the ginseng root. In our study, we first extracted the ginsenosides from the roots of commercially grown American ginseng and then used reversed-phase liquid chromatography (RPLC) to separate and isolate the components. We screened several column types using high and low pH as well as methanol and acetonitrile to develop an optimized methodology. We know that more than 30 components have been found in ginseng roots, and we identified the most enriched components using both HPLC-mass spectrometry (MS) and HPLC-evaporative light scattering detection (ELSD). The analytical method was scaled up to preparative chromatography. Recoveries and purity were measured to demonstrate the effectiveness of the sample preparation and purification method. These results indicate that effective method development, multiple detection methods and chromatographic instrumentation are all important parameters in obtaining an optimized purification process.

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