Analysis of Human Saliva by Intact Protein LC/MS: Reproducibility, Variability and PTM Characterization

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Kevin M. Millea, Ira S. Krull (Northeastern University);Asish B. Chakraborty, Ignatius J. Kass, John C. Gebler, Scott Berger (Waters)
HUPO 2006 March 12-15, 2006 Boston, MA; HUPO 2006 October 28 - November 1 Long Beach, Ca
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Intact protein LC/MS analysis can yield analytically reproducible results for a given saliva sample. Phosphoproteins could be easily identified by treatment with alkaline phosphatase. Individuals were observed to have a similar salivary protein pattern over the course of several days, although intensities of the observed proteins were variable. More significant differences in protein pattern were observed between individual subjects.The lack of strong salivary homeostatic mechanisms may explain the observed differences between samples, and could complicate proteomic or biomarker investigations using saliva samples.The need for larger and better controlled studies of intrasubject and inter-subject saliva protein variation are indicated.

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