Phosphopeptide Enrichment for Mass Spectrometry Analysis Using Microscale Titanium Dioxide Solid Phase Extraction
ASMS 2006, Phosphopeptide Enrichment _TiO2 and IMAC were
compared in terms of selectivity and recovery for phosphopeptide
enrichment using a standard Enolase peptide/Phosphopeptide mixture.This
standard mixture sample allowed us to perform unbiased comparison of
different phosphopeptide enrichment devices. The SPE protocol for TiO2
involves less steps than IMAC since metal chelation is not required.
For TiO2:The highest recovery from these loading conditions is with the
0.1TFA in high organic.Loading samples mixed with an unique reagent,
Enhancer™, ontoTiO2 SPE improves the phosphopeptide selectivity
significantly. Additives, such as detergents and salts, at low levels
do not alterthe mechanism of action for TiO2, unlike IMAC, and can
additionally be used as a sample clean-up device.