Qualitative/Quantitative Analysis of E.Coli by Time-Resolved Mass Spectrometry

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Jeffrey C. Silva, Richard Denny, Craig Dorschel, Guo-Zhong Li, Marc V. Gorenstein, Keith Richardson, Daniel B. Wall, Scott J. Geromanos, and David Heywood
International Canadian Proteomics Initiative May 10-12, 2006 Edmonton, Alberta, Canada; Brazilian Society of Biochemistry and Molecular Biology, July 1 - 4, 2006, Sao Paulo, Brazil
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A parallel LC/MS method (LCMSE) was used to simultaneously quantify and identify proteins involved in carbon source utilization. The parallel LC/MS method provides a means to obtain time-resolved accurate mass measurements for precursors and fragments. Protein identifications from several metabolic pathways of E. coli were made using the accurate mass measurements of the detected precursors and their associated fragments. A label-free quantitation method was used to determine the relative levels of these proteins among the different growth conditions. Results are consistent with those obtained by other methods.

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