How to Reduce Sample Complexity For MALDI Analysis: Optimization of Off-line LC MALDI Analysis

Library Number:
720001340EN
Part Number:
720001340EN
Author(s):
Emmanuelle Claude, J. Rontree, M. Kennedy, M. Snel, R. O’Malley, J. Langridge
Source:
Waters Poster
Content Type:
Posters
Content Subtype:
Other Symposium
Related Products:
Described in this poster is the coupling of nanoACQUITY UPLC™ system to MALDI micro MX™ and MALDI Q-Tof™ instruments. Evaluation of the system sensitivity and resolving power was performed. The glycosylated recombinant protein Erythropoietin (EPO) was tryptically digested and analyzed as an unseparated mixture and subsequently by LC-MALDI. The Conclusions of this poster are: A new combination of nanoACQUITY UPLC with MALDI spotting and MALDI analysis (axial MALDI TOF and MALDI Q-TOF) was evaluated. Sensitivity: 5 fmol of ADH digest on column was successfully separated and analyzed. Chromatographic resolution: Similar chromatographic resolution vs on-line LC-MS was observed, (ca 20 sec peak width at base) LC-MALDI identified 62 additional peptides from an EPO tryptic digest Two O-linked glycopeptides from two the different glycoforms of EPO were analyzed by LC-MALDI MS/MS

Title Format File Size
720001340en PDF 293.87kB