Rapid Candida Species Identification- Is MALDI-TOF MS The Answer?

Library Number:
Part Number:
H. Sutton1, D. Dare1, S. Clements2, C. Kibbler2, E. Johnson3, M. Lunt4, H. Shah5. 1.Manchester Metropolitan University, Manchester, UK, 2.The Royal Free Hospital, London, UK, 3.NCPF Bristol, Bristol, UK, 4.Waters Corporation, Manchester, UK, 5.Health Protection Agency, London, UK.
IUMS 2005
Content Type:
Content Subtype:
The incidence of superficial and deep fungal infections have been on the increase over the last decade, so much so that Candida species are now the fourth most common cause of bloodstream infections in the United States, it is paramount in these cases that a rapid identification is possible to ensure an appropriate effective course of treatment is made available to the patient. Traditional fungal and yeast identification methods usually involve biochemical, morphological and physiological tests, that can be time consuming and labour intensive. These techniques also require a high level of skill, training and often the personal judgement/experience of a clinical mycologist. Commercial kits are available for the more clinically relevant yeasts and fungi including Candida species, these however are mainly based on biochemical tests and/or colour interpretation, often only covering a narrow range of species and/or require a 24-72h following culture to obtain an identification.

MALDI-TOF MS has recently shown great success in bacterial identification and this paper aims to explore the possibility of developing the technique to include yeasts, in particular Candida species. The use of MALDI-TOF MS has the advantage of reducing culture time to 24 hours, and identification to within minutes.

Title Format File Size
wa41934 PDF 457.69kB