Effect of Sample Preparation and Chromatographic Separation on Matrix Effects in Quantitative Bioanalysis

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Iain Gibb, Ed Sprake, Steve Preece (Waters, Manchester, UK) Erin McManus, Diane Diehl (Waters, Milford, MA, USA)
British Mass Spec Society Meeting, BMSS
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Other Symposium
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The MS response obtained from an analyte solution can differ significantly from that same analyte in a matrix due to ion suppression and interference from co-eluting metabolites and matrix components. Ion suppression results from co-eluting components that compete for ionization capacity and cause errors in accuracy and precision. Metabolite effects occur when a drugrelated compound co-elutes with the test compound and then fragments in the ion source of the mass spectrometer to give a product that interferes with the MRM channel for the drug. This will lead to an elevated response for the test compound. These effects can sometimes be addressed by redeveloping the chromatography, but this often increases sample analysis time. Matrix effects should be evaluated as a part of quantitative LC/ESI-MS/MS method development and validation. Here we investigate the use of mixed-mode solid phase extraction and efficient chromatographic techniques to reduce matrix effects for a range of analytes.

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