Improvements in Hybrid Particle Technology: A New Family of Hybrid Packing Materials for Reversed-Phase HPLC

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Erin Chambers, Pamela Iraneta, Bonnie Alden, Diana Swanson, John O’Gara, Kevin Wyndham, Darryl Brousmiche, Nicole Lawrence, Kevin Jenkins, Diane Diehl, Jeffrey Mazzeo[Waters]
Pittcon, March13-16, 2006
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The first commercially available hybrid organic/inorganic packing materials were introduced in 1999 as the XTerra® family of reversed-phase HPLC columns. Continued improvements in particle design resulted in a new particle that contains bridged ethyl groups in place of the methyl groups of the first generation hybrid. This substantially improved brand of reversed-phase hybrid columns was introduced last year: the XBridge™ family of columns. We will show comparisons of peak shape, efficiency and lifetime between these new columns and top commercially available columns. Unlike commonly used bonding approaches, our improved bonding technology confers excellent stability in low and high pH mobile phases. The presented data supports the use of several XBridge stationary phases across an expanded pH range. An additional benefit of these new columns is the exceptional stability across the pH 2 to pH 12 buffering range of phosphate. Traditional silica-based columns and even extended pH columns do not last for more than 50 injections with pH 12 phosphate buffer. The XBridge family of columns exhibits at least an order of magnitude improvement in stability, providing method developers with added mobile phase flexibility. We will also present data outlining how these new columns can be used to efficiently develop methods for active pharmaceutical ingredients and their impurities.

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