A Method for Estimating the Absolute Concentration of Proteins in Complex Mixtures

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Jeffrey C. Silva, Craig A. Dorschel, Martin Gilar, Marc V. Gorenstein, Petra Olivova, Johannes P. C. Vissers, Guo-Zhong Li and Scott J. Geromanos (Waters)
International Canadian Proteomics Initiative 2006, May 10-12, 2006, Edmunton, Alberta, Canada; Beyond Genome, San Fransisco, Ca; Brazilian Society of Biochemistry and Molecular Biology, July 1 - 4, 2006, Sao Paulo, Brazil
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This poster describes a new method of absolute quantification of proteins based on the discovery of a relationship between MS signal response and the absolute protein concentration, wherein the average MS signal response for the three most intense tryptic peptides is constant per mole of protein.

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