Exploiting Accurate Mass Information for Metabolite Structure Elucidation Using a Data Independent Strategy.
Finding and identifying metabolites in cold in vivo studies by
LC-MS is extremely challenging, as metabolites are often present at
such low levels that chromatographically they are indistinguishable
from the background endogenous materials. In practice this means
several LC-MS-(MS) experiments are usually performed for each sample to
fully explain the metabolic fate of the compound. In this work we
describe a novel approach that enables all of the key metabolite
information to be collected in a single LC/MS run.