Increased Selectivity for the Ion Mapping of Synthetic Antagonists of Dopamine Receptors in Rat Brain Specimens Using MS/MS on a MALDI Q-Tof MS

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Marten Snel, Steve Wilson, Daniel Kenny, Emmanuelle Claude, Richard Tyldesley-Worster, James Langridge
ANZSMS 2007, New Zealand, January 22
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Imaging the spatial distribution of molecules in tissue using MALDI mass spectrometers is a rapidly developing technique. The acquisition of accurate mass data in this type of experiment can be hampered in axial MALDI Tof systems. Even small changes in sample position and laser energy in the source region of this type of mass spectrometer affect mass measurement accuracy and mass spectral resolution. The use of an orthogonal TOF MALDI mass spectrometer circumvents these problems by decoupling the MALDI source from the mass analyser. Raclopride is retained in tissue as a result of binding to a neurotransmitter receptor.  Raclopride is a selective dopamine antagonist with a high affinity for dopamine type 2 (D 2) receptors. After intravenous administration, raclopride localises in the basal ganglia, a region with a high density of dopamine receptors. PET images show stereoselective concentration of raclopride in the region of the putamen relative to the rest of the brain2. Data obtained on the spatial distribution of Raclopride and endogenous adenosine monophosphate are shown here.

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