Exploring the Use of Elevated Temperature for UPLC Applications

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Michael D. Jones, Paul Rainville, Andrew Aubin, Eric Grumbach, Robert Plumb, Jeff Mazzeo
HPLC 2007, June 17-21, Ghent, Belgium
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This experiment explores the use of elevated temperature in liquid chromatography. As the temperature of the mobile phase is increased, several variables that play a key role in chromatographic performance are affected. Mobile phase viscosity is decreased at higher temperatures. This means that with a constant mobile phase, flow rate, column dimension and particle size, operating pressure is reduced at higher temperature. Analyte diffusivity is increased at higher temperature. This fact results in a shift in the optimum flow rate to higher values.

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