Structural Characterization of a Recombinant Monoclonal Antibody by Electrospray Ion Mobility (IMS) TOF MS
A new class of mass spectrometer has been developed by Waters (Synapt HDMS) with the unique capability to fractionate biomolecules in the gas phase by size, shape, and charge prior to mass spectrometric detection. The Synapt HDMS system enables researchers to simplify complex samples, simplify or eliminate chromatographic separations, or permit the indepth characterization of hybrid structures (e.g. glycopeptides) within a single analysis. This poster presents the design and theory behind Synapt HDMS, and shows specific applications to the analysis of subunits of monoclonal immunoglobulins, glycoproteins, and the complex peptide and glycan mixtures resulting from these biomolecules.