Investigation of High m/z species using ion-mobility MS

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Iain Campuzano, Kevin Giles, James Langridge, Albert Heck, and Cees Versluis
British Mass Spectroscopy Society, September 9 - 12, Heriot Watt University, Edinburgh
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Over the past 10 years interest in high mass non-covalent protein analysis has increased due to the ability of the current mass spectrometers and electrospray sources to preserve the non-covalent protein/protein subunit interactions, allowing one to analyse proteins in their native conformation and stoichiometry. The transfer of non-covalently associated protein-protein complexes from solution to the gas phase using electrospray ionisation generally results in the formation of ions possessing relatively few charges.  As a result the m/z values can be quite large, making mass analysis using time-of-flight instrumentation amongst the most efficient. The utility of ion mobility separation (IMS) in probing the structures of relatively large complexes has been highlighted previously.  Here we report further evaluation of a hybrid ion mobility / time-of-flight instrument for analysis of high m/z species, including the 800kDa GroEL complex, under non-activation and collisional activation conditions.

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