Inorganic-Organic Hybrid Phases- Combining the Best of Both Worlds

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Uwe D. Neue;Jeffrey R. Mazzeo;Diane M. Diehl;KimVan Tran;Eric Grumbach;Alberto Mendez;Pamela C. Iraneta;Kevin D. Wyndham;John c. O''Gara;Thomas H. Walter [Waters]
Pittcon 2003
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Diuretics; Triamterene; Furosemide; Bumetanide; Benzthiazide; Chlorthalidone; Althiazide; Ethacrynic acid; Probenecid; Canrenoic acid; Uracil; Propranolol; Butylparaben; Dipropylphthalate; Naphthalene; Acenaphthene; Amitryptyline
XTerra RP18 All Sizes and Dimensions XTerra MS C18 All Sizes and Dimensions
Standard silica-based bonded phases for HPLC can only be used over a limited pH range. Polymeric phases, such as divinyl-benzene phases, do not have this problem, but suffer from inferior mass-transfer properties. In our research we have attempted to combine the best properties of inorganic packings and polymeric packings. To this purpose, we have studied inorganic- organic hybrid phases. Various options are available to design new hybrid phases. We will describe the characteristics of these phases in detail. This discussion includes the retention properties, mass-transfer behavior and, most importantly, the pH and temperature stability of these phases. In addition, we show application examples that take advantage of the expanded temperature and pH range of these packings (1, 2). An examination of these application examples allows us also to judge the impact of the broad temperature and pH range on method development (1). The combination of optimized surface chemistries together with new options in method development is particularly attractive to the HPLC user, since it permits the speedy creation of new HPLC methods.

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